School of Medicine

Wayne State University School of Medicine

Project Submission

Sanger DNA Sequencing

For samples requiring Sanger DNA sequencing ONLY, click here.

Project Consultation

For projects requiring other than just DNA sequencing, the first step is to contact the facilities Director, Dr. Sue Land, in order to set up a project consultation.  During this consultation, the project-at-hand can be mapped out from start to finish.  All aspects of the project including, but not limited to, project design, sample requirements, price estimates, turnaround time, bioinformatics, etc., will be discussed.

Sample Submission

Before human or animal samples can be submitted to the facility, one must have current IRB approval from the human and/or animal committee.

When samples are submitted to the facility, it is critical that they be properly labeled with a legible sample identifier.  Sample requirements (ie. appropriate vessel, amount of starting material, storage provisions, etc.) can vary from one platform to the next, therefore, these requirements are discussed at length during the project consultation. 

Please note, the C.S. Mott Center which houses our facility is a secure building.  The appropriate AGTC staff member (as determined during project consultation) must be contacted in order to arrange for sample delivery.

Requesting AGTC Services in iLabs

All AGTC services must be requested in iLabs https://waynestate.corefacilities.org/service_center/show_external/2923

If you need assistance registering with iLabs, please contact Ginette Borovicka.

Log in to iLabs and navigate to the AGTC core page. Select the AGTC Service Request tab. A list of our areas of service will be presented to you. Find the link to the service you require and proceed. Please ensure that you are requesting services under the correct financial account information for your submission. Use the Project Description window to enter the project details (ie. type of sample, number of samples, etc.) Please include as much information as possible. You also have an option to upload a pre-written description of your project, samples, and required services.We strongly recommend visiting the service-specific sections of the AGTC website and consulting with the appropriate staff member(s) prior to requesting services. Additional information regarding iLabs can be found through the links below:

Internal (WSU) Users: An estimated cost for requested services will be entered and submitted for your approval prior to sample submission. Once projected costs are approved by the researcher, the AGTC will initiate sample processing.

External Users: Following approval of the projected cost, a copy of a valid Purchase Order must be uploaded to your project page in iLabs before the AGTC will proceed with sample processing.

Estimates

Most AGTC service categories include an option for requesting an estimate. This option should only be selected for projects that will not be ready for submission within 30 days of the request, or if an estimate is required for a future grant submission. Once the estimate has been generated, the researcher is required to approve it in iLabs, confirming that it has been received, and the estimate service will be completed.

Note: The researcher will not be billed for the estimate service. Estimates are subject to change.

Required Documentation

Specific instructions for sample submission and documentation is available in the relevant section of the AGTC website, and will be clarified during the consultation process. General requirements are listed below:

DNA projects:

  1. When submitting samples for DNA isolation, please click here for required documentation.
  2. When submitting DNA extracts, the AGTC DNA Sample Submission Form must be completed and uploaded into iLabs when requesting any DNA isolation service.
  3. For all genotyping projects, the AGTC Genotyping Project Submission Form must be completed and uploaded into iLabs when requesting any genotyping services.

RNA projects:

  1. For samples being submitted for RNA isolation, RNA Quality Assessment, or Gene Expression Microarrays, please refer to their respective sections of the AGTC website. If you require further information or consultation, please contact Dan Lott
  2. For project and sample submission information regarding Next generation Sequencing (NGS), please contact Dr. Sue Land

Turnaround Time

The facility makes every effort to complete projects as quickly as possible.  However, processing times will vary depending on current AGTC workload, project scope, platform, and product availability.

Project Completion

Once a project is completed, the investigator will be contacted in order to arrange for pick up of any remaining samples.  In addition, we would greatly appreciate acknowledgment in any published work.