School of Medicine

Wayne State University School of Medicine

Methylation

General Information

The Applied Genomics Technology Center provides the following DNA methylation services:

Note: Illumina is discontinuing the Infinium Human Methylation 450K BeadChip effective in December, 2015. Please refer to the following notice for information and alternative products.

Sample Requirements

All the sample requirements listed below are guidelines established by the various vendors.  The AGTC staff will work with investigators to optimize these assays for their specific sample types. 

DNA methylation profiling using Illumina Infinium® Assay Technology 

  • Submit 25ul of DNA at a concentration of 50ng/ul
  • The bisulfite conversion process requires 1ug of DNA in a specific volume, therefore the concentration listed above is necessary

   

Requesting AGTC Services in iLabs

All AGTC services must be requested in iLabs https://waynestate.corefacilities.org/service_center/show_external/2923

If you need assistance registering with iLabs, please contact Ginette Borovicka.

Log in to iLabs and navigate to the AGTC core page. Select the AGTC Service Request tab. A list of our areas of service will be presented to you. Find the link to the service you require and proceed. Please ensure that you are requesting services under the correct financial account information for your submission. Use the Project Description window to enter the project details (ie. type of sample, number of samples, etc.) Please include as much information as possible. You also have an option to upload a pre-written description of your project, samples, and required services.We strongly recommend visiting the service-specific sections of the AGTC website and consulting with the appropriate staff member(s) prior to requesting services. Additional information regarding iLabs can be found through the links below:

Internal (WSU) Users: An estimated cost for requested services will be entered and submitted for your approval prior to sample submission. Once projected costs are approved by the researcher, the AGTC will initiate sample processing.

External Users: Following approval of the projected cost, a copy of a valid Purchase Order must be uploaded to your project page in iLabs before the AGTC will proceed with sample processing.

Estimates

Most AGTC service categories include an option for requesting an estimate. This option should only be selected for projects that will not be ready for submission within 30 days of the request, or if an estimate is required for a future grant submission. Once the estimate has been generated, the researcher is required to approve it in iLabs, confirming that it has been received, and the estimate service will be completed.

Note: The researcher will not be billed for the estimate service. Estimates are subject to change.

Required Documentation

Specific instructions for sample submission and documentation is available in the relevant section of the AGTC website, and will be clarified during the consultation process.

Additional Information

If needed, please contact Nicole Ducharme for further information reguarding our DNA methylation services.