The AGTC DNA Sequencing Lab provides fast, reliable and accurate DNA sequencing for a variety of sample types including plasmid DNA as well as PCR product. The Sequencing Lab is equipped with an Applied Biosystems ABI Prism 3730 DNA Analyzer, which is able to sequence as many as 96 samples per sequencing plate and over 570 samples per day. The DNA sequencing is accomplished using the ABI BigDye® Terminator v3.1 Cycle Sequencing Kit. With the BigDye® Terminator v3.1 chemistry, you get robust, highly flexible chemistries for a wide range of applications, including de novo sequencing and resequencing.

We offer the investigator two options for sequencing: you can perform your own sequencing reactions with the ABI BigDye® Terminator v3.1 Cycle Sequencing Kit or the reactions will be done by our staff using the ABI BigDye® Terminator v3.1 chemistry. For required sample concentrations and quantities, please click here.

The researcher can submit his/her own custom-designed primers (see primer concentrations for details) or use our common primers ((T7, T3, SP6, M13 Forward & M13 Reverse) provided by the Sequencing Lab at no additional charge. For required primer concentrations and quantities, please click here.

Submitting DNA samples for sequencing:

Sequencing request are made through our on-line DNA Sequencing Submission website, please click here to submit a request.

Sequencing results will be sent electronically to your email account or to our FTP site (for large sets of data). If requested, a paper hard-copy of the chromatogram can be sent for a nominal charge.

DNA Sequencing Pricing: 

For our current DNA Sequencing pricing, please click here.

 If you have any questions or comments, please email us at weseqdna@wayne.edu or call us at (313) 577-0024.