School of Medicine

Wayne State University School of Medicine

DNA Isolation

General Information

The Applied Genomics Technology Center (AGTC) has vast experience with genomic DNA isolation from a variety of sample sources including whole blood, blood clots, dried bloodspots, buffy coats, plasma, serum, urine, saliva, mouthwashes, buccal swabs, cultured cells, fresh/frozen tissues, and paraffin-embedded tissues. We offer a great variety of methodologies to our investigators which allows for successful genomic DNA isolation from even the most challenging of starting materials.

The DNA Isolation Lab is equipped with two state-of-the-art, automated, nucleic acid isolation systems: The EZ1® Advanced and the QIAsymphony SP.  Automated systems allow greater yield and uniformity as well as a decrease in turnaround time.   

Requesting AGTC Services in iLabs

All AGTC services must be requested in iLabs https://waynestate.corefacilities.org/service_center/show_external/2923

If you need assistance registering with iLabs, please contact Ginette Borovicka

Log in to iLabs and navigate to the AGTC core page. Select the AGTC Service Request tab. A list of our areas of service will be presented to you. Find the link to the service you require and proceed. Please ensure that you are requesting services under the correct financial account information for your submission. Use the Project Description window to enter the project details (ie. type of sample, number of samples, etc.) Please include as much information as possible. You also have an option to upload a pre-written description of your project, samples, and required services.We strongly recommend visiting the service-specific sections of the AGTC website and consulting with the appropriate staff member(s) prior to requesting services. Additional information regarding iLabs can be found through the links below:

Internal (WSU) Users: An estimated cost for requested services will be entered and submitted for your approval prior to sample submission. Once projected costs are approved by the researcher, the AGTC will initiate sample processing.

External Users: Following approval of the projected cost, a copy of a valid Purchase Order must be uploaded to your project page in iLabs before the AGTC will proceed with sample processing.

Estimates

Most AGTC service categories include an option for requesting an estimate. This option should only be selected for projects that will not be ready for submission within 30 days of the request, or if an estimate is required for a future grant submission. Once the estimate has been generated, the researcher is required to approve it in iLabs, confirming that it has been received, and the estimate service will be completed.

Note: The researcher will not be billed for the estimate service. Estimates are subject to change.

Sample Submission

Sample requirements are striclty dependent on the type of sample, the DNA isolation platform, and the intended downstream applications . Please contact the AGTC prior to collecting and submitting samples to confirm these requirements.

When submitting samples for DNA Isolation, the AGTC DNA Isolation Sample Submission Form must be filled out in its entirety and sent via email to the AGTC (there are multiple worksheet tabs at the bottom of the form) or uploaded into your iLabs service request.  Following review of the submitted sample submission form, sample drop off will be authorized and coordinated.