RNA Quality Assessment

The AGTC assesses RNA quality using the RNA 6000 Nano Assay for the Agilent 2100 Bioanalyzer. The assay generates an electrophoretogram of the total RNA fragments, a 28S/18S ratio, and an RNA Integrity Number (RIN) which are collectively used to determine the overall quality. This service is also available in conjunction with RNA isolation services and microarray sample processing.

Sample Requirements

RNA samples submitted specifically for RNA quality assessment should be prepared as purified total RNA in nuclease-free water. The AGTC requires 5 microliters (ul) of each sample at an adjusted concentration within the range 50 to 500 ng/ul. An AGTC project submission form and a full sample list prepared in MS Excel must be emailed to Daniel Lott prior to sample submission.  All samples must be stored at -80°C and transported to the AGTC on dry ice.

RNA samples submitted for RNA quality assessment in conjunction with microarray processing will be subject to the sample requirements dictated by the specific intended microarray platform.

Additional Information

If needed, please contact Daniel Lott for further information regarding RNA isolation.