General Information

The Applied Genomics Technology Center is equipped with an Illumina Genome Analyzer II with Paired-end module for next generation sequencing. Applications include Genome Sequencing, Transcriptome Analysis, and Gene Regulation & Epigenetic Analysis.

Current Services

• Genomic DNA resequencing and De novo sequencing
• mRNA seq 
• Small RNA discovery and analysis
• ChIP seq
• Methylation analysis

Capabilities

• 8 samples per flowcell
• PhiX control DNA available
• 18, 36, or 76 cycles in single read or paired end run
• Multiplexing up to 12 samples in one lane (using Illumina indexing sequences)

Sample Requirements

For most applications 5 µg DNA/RNA is required. ChIP seq samples require a minimum of 100ng DNA from chromatin immunoprecipitation.

Please note that it may take 2-3 weeks to receive sample prep kits from Illumina.

Additional Information

If needed, please contact Melissa Coon for further information regarding the Genome Analyzer II.